Resolution
Use Ernst Abbes famous formula to characterize microscope objective lenses.
Characterize your microscope objective lenses, see how well matched they are to your microscopes image acquisition system. Input the magnification, immersion medium (air, oil, glycerol or silicone oil), NA (numerical aperture) and wavelength of light (lambda), to calculate the lateral resolution, axial resolution and fluorescence brightness of the objective. Characterize your acquisition system (camera pixel size, camera binning and additional magnification) to check if youre sampling at Nyquist frequency. Each of your objectives can be saved and easily restored, and shared with other microscopists via e-mail, MMS, Linked-In or even Facebook (why not?). The equations used for the calculations can be displayed by selecting the "Show Equations" option from the App bar menu. The sources of equations are referenced in the "About" box.* Theoretical Resolution is calculated according Ernst Abbes famous equation (Abbe 1873)* Actual Resolution is calculated according to ‘The Rayleigh Criterion’* Axial Resolution is calculated according to Inoues chapter in Jim Pawleys “Handbook of Biological Confocal Microscopy” (3rd edition 2006)* Camera pixel size can be loaded from the list of cameras, if your camera is not in the list, you can enter it manually in the Camera dialog.* Select the "share" icon to share all output to e-mail, MMS or Linked-In* Access features from the App Bar Menu (the "three dots" icon - top right)* Click on the "Lambda" or "NA" text fields to open dialogs that directly set these values * It doesnt matter if your microscope was made by Zeiss, Evos, Leica, Olympus, Nikon, Motic, or Meiji Techno, the principles are identical and you will find the app useful.* The list of cameras in the Camera dialog includes those made by Andor, Hamamatsu, Photometrics, PCO, QImaging, Leica, Olympus, Nikon, and Zeiss.Ive tried to improve the layout on phones with large screensAdded the Andor Sona cameras. Added the Leica K5.
Characterize your microscope objective lenses, see how well matched they are to your microscopes image acquisition system. Input the magnification, immersion medium (air, oil, glycerol or silicone oil), NA (numerical aperture) and wavelength of light (lambda), to calculate the lateral resolution, axial resolution and fluorescence brightness of the objective. Characterize your acquisition system (camera pixel size, camera binning and additional magnification) to check if youre sampling at Nyquist frequency. Each of your objectives can be saved and easily restored, and shared with other microscopists via e-mail, MMS, Linked-In or even Facebook (why not?). The equations used for the calculations can be displayed by selecting the "Show Equations" option from the App bar menu. The sources of equations are referenced in the "About" box.* Theoretical Resolution is calculated according Ernst Abbes famous equation (Abbe 1873)* Actual Resolution is calculated according to ‘The Rayleigh Criterion’* Axial Resolution is calculated according to Inoues chapter in Jim Pawleys “Handbook of Biological Confocal Microscopy” (3rd edition 2006)* Camera pixel size can be loaded from the list of cameras, if your camera is not in the list, you can enter it manually in the Camera dialog.* Select the "share" icon to share all output to e-mail, MMS or Linked-In* Access features from the App Bar Menu (the "three dots" icon - top right)* Click on the "Lambda" or "NA" text fields to open dialogs that directly set these values * It doesnt matter if your microscope was made by Zeiss, Evos, Leica, Olympus, Nikon, Motic, or Meiji Techno, the principles are identical and you will find the app useful.* The list of cameras in the Camera dialog includes those made by Andor, Hamamatsu, Photometrics, PCO, QImaging, Leica, Olympus, Nikon, and Zeiss.Ive tried to improve the layout on phones with large screensAdded the Andor Sona cameras. Added the Leica K5.
Read trusted reviews from application customers
Top and acceptable
Great app Andrew very helpful. Thanks for taking the time to create this.
Very handy tool for microscopists!!
Super easy to use and extremely useful. A must for every microscope user.
this is awesome!
Because when I read the reviews the developer answered on a bunch of reviews I thought it was cool he was so involved in his work
I'm an idiot.
Looks so sophisticated and complex .I think the help needs more help
Bakwas app
Almost perfect for my use. Further improvements can be made by adding more objective magnifications, such as my 65x, 70x, 80x and 90x (LOMO water and oil objectives); that way the relative (objective) brightness calculation can be done correctly. Also, pairs of brackets are missing in resolution equations.